NDRG1 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Protein NDRG1, Differentiation-related gene 1 protein, DRG-1, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, Reducing agents and tunicamycin-responsive protein, RTP, Rit42, NDRG1, CAP43, DRG1, RTP | | Тип антител | Recombinant Antibody | | Species | Human | | UniProt ID | Q92597 | | Иммуноген | A synthesized peptide derived from human NDRG1 | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 | | Клональность | Monoclonal | | Изотип | Rabbit IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Affinity-chromatography | | Области исследований | Neuroscience | | Аббревиатура | Protein NDRG1 | | Примечание | Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy. | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate, Mouse kidney tissue
All lanes: NDRG1 antibody at 0.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 36, 34 KDa
Observed band size: 43 KDa
 | IHC image of CSB-RA835678A0HU diluted at 1:71.6666666666667 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunofluorescence staining of Hela cells with CSB-RA835678A0HU at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Наименование: NDRG1 Antibody.