NEDD4L AntibodyСпецификация| Объем | 50 мкг | | Синонимы | E3 ubiquitin-protein ligase NEDD4-like (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase NED4L) (NEDD4.2) (Nedd4-2), NEDD4L, KIAA0439 NEDL3 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q96PU5 | | Иммуноген | Recombinant Human E3 ubiquitin-protein ligase NEDD4-like protein (289-494AA) | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 | | Примечание | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, KCNH2, EAAT1 or CLC5 (PubMed:26363003). Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046). Plays a role in dendrite formation by melanocytes (PubMed:23999003). Involved in the regulation of TOR signaling (PubMed:27694961). | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | E3 ubiquitin-protein ligase NEDD4-like | | Области исследований | Epigenetics and Nuclear Signaling, Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Hela whole cell lysate
All lanes: NEDD4L antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 112, 105, 101, 99, 111, 110, 97 kDa
Observed band size: 112 kDa
 | Immunofluorescence staining of HepG2 cells with CSB-PA850411LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: NEDD4L Antibody.