NFKBIE (Ab-22) AntibodyСпецификацияОбъем | 100 мкл | Синонимы | I-kappa-B-epsilon | Клональность | Polyclonal Antibody | Организм | Human | uniprot | O00221 | Иммуноген | Peptide sequence around aa. 20~24 (I-E-S-L-R) derived from Human IkB-e. | Источник | Rabbit | Видовая специфичность | Human, Mouse | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | Примечание | Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.<br/><br/>Shirane M, et al. (1999) J Biol Chem, 274(40): 28169-74<br/>Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ,18, 621-663.<br/>Chen, Z.J. et al. (1996) Cell, 84, 853-862.<br/>Brown, K. et al. (1995) Science, 267, 1485-1488.<br/> | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | Форма | liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | Области исследований | Epigenetics and Nuclear Signaling,Immunology,Signal transduction | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from Hela, 293 and HepG2 cells using IkB-e(Ab-22) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-e(Ab-22) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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