Объем | 100 мкл |
Синонимы | ERK activator kinase 2, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P36507 |
Иммуноген | Peptide sequence around aa.392~396 (P-G-T-P-T) derived from Human MEK-2. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 |
Примечание | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases <br/><br/>Crews C M, et al. (1992) Science. 258:478-480.<br/>Alessi D R, et al. (1994) EMBO J. 13:1610-1619.<br/>Rosen L B, et al. (1994) Neuron. 12:1207-1221.<br/>Cowley S, et al. (1994) Cell. 77:841-852.<br/> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
Области исследований | Cancer,Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela, HepG2, PC12 and HUVEC cells using MEK-2(Ab-394) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2(Ab-394) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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