| Объем | 50 мкг |
| Синонимы | Dual specificity mitogen-activated protein kinase kinase 5 (MAP kinase kinase 5) (MAPKK 5) (EC 2.7.12.2) (MAPK/ERK kinase 5) (MEK 5), MAP2K5, MEK5 MKK5 PRKMK5 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13163 |
| Иммуноген | Recombinant Human Dual specificity mitogen-activated protein kinase kinase 5 protein (1-190AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:200-1:500 |
| Примечание | Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Dual specificity mitogen-activated protein kinase kinase 5 |
| Области исследований | Cancer, Cardiovascular, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, Rat liver tissue, Mouse liver tissue
All lanes: MAP2K5 antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51, 49, 50, 47 kDa
Observed band size: 51 kDa
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Immunofluorescence staining of Hela cells with CSB-PA615667HA01HU at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: MAP2K5 Antibody.