| Объем | 50 мкг |
| Синонимы | Dual specificity mitogen-activated protein kinase kinase 7 (MAP kinase kinase 7) (MAPKK 7) (EC 2.7.12.2) (JNK-activating kinase 2) (MAPK/ERK kinase 7) (MEK 7) (Stress-activated protein kinase kinase 4) (SAPK kinase 4) (SAPKK-4) (SAPKK4) (c-Jun N-terminal kinase kinase 2) (JNK kinase 2) (JNKK 2), MAP2K7, JNKK2 MEK7 MKK7 PRKMK7 SKK4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O14733 |
| Иммуноген | Recombinant Human Dual specificity mitogen-activated protein kinase kinase 7 protein (17-160AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200 |
| Примечание | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Dual specificity mitogen-activated protein kinase kinase 7 |
| Области исследований | Immunology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescent analysis of PC-3 cells using CSB-PA013416LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
 |
Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate
All lanes: MAP2K7 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 52, 50, 49 kDa
Observed band size: 48 kDa
 |
| | |
Наименование: MAP2K7 Antibody.