| Объем | 50 мкг |
| Синонимы | Mitogen-activated protein kinase 13 (MAP kinase 13) (MAPK 13) (EC 2.7.11.24) (Mitogen-activated protein kinase p38 delta) (MAP kinase p38 delta) (Stress-activated protein kinase 4), MAPK13, PRKM13 SAPK4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O15264 |
| Иммуноген | Recombinant Human Mitogen-activated protein kinase 13 protein (1-365AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cell biology, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: MAPK13 antibody at 2µg/ml
Lane 1: Hela cells
Lane 2: A549 cells
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43, 29 kDa
Observed band size: 43 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA013452LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA013452LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA013452LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: MAPK13 Antibody.