| Объем | 100 мкг |
| Синонимы | Epididymis-specific alpha-mannosidase (EC 3.2.1.24) (Mannosidase alpha class 2B member 2), MAN2B2, KIAA0935 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9Y2E5 |
| Иммуноген | Recombinant Human Epididymis-specific alpha-mannosidase protein (347-523AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
| Примечание | extracellular exosome, lysosomal lumen, alpha-mannosidase activity, mannan endo-1,6-alpha-mannosidase activity, mannose metabolic process, oligosaccharide catabolic process, protein deglycosylation |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Epididymis-specific alpha-mannosidase |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Rat kidney tissue
All lanes: MAN2B2 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 114, 108 kDa
Observed band size: 114 kDa
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Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate
All lanes: MAN2B2 antibody at 1.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 114, 108 kDa
Observed band size: 135 kDa
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IHC image of CSB-PA896489LA01HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: MAN2B2 Antibody.