Объем | 100 мкг |
Синонимы | Melanocyte-stimulating hormone receptor (MSH-R) (Melanocortin receptor 1) (MC1-R), MC1R, MSHR |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q01726 |
Иммуноген | Recombinant Human Melanocyte-stimulating hormone receptor protein (1-37AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Примечание | Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Melanocyte-stimulating hormone receptor |
Области исследований | Neuroscience, Metabolism |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: A375 whole cell lysate
All lanes: MC1R antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
![](http://www.cusabio.cn/images/antibody/4/CSB-PA013558LA01HU-WB.jpg) |
IHC image of CSB-PA013558LA01HU diluted at 1:400 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
![](http://www.cusabio.cn/images/antibody/4/CSB-PA013558LA01HU-IHC-1.jpg) |
IHC image of CSB-PA013558LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
![](http://www.cusabio.cn/images/antibody/4/CSB-PA013558LA01HU-IHC-2.jpg) |
Immunofluorescence staining of Hela cells with CSB-PA013558LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
![](http://www.cusabio.cn/images/antibody/5/CSB-PA013558LA01HU-IF.jpg) |
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