Объем | 50 мкг |
Синонимы | MKL/myocardin-like protein 2 (Megakaryoblastic leukemia 2) (Myocardin-related transcription factor B) (MRTF-B), MKL2, KIAA1243 MRTFB |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9ULH7 |
Иммуноген | Recombinant Human MKL/myocardin-like protein 2 protein (164-313AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000 |
Примечание | Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | MKL/myocardin-like protein 2 |
Области исследований | Epigenetics and Nuclear Signaling, Cardiovascular, Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate
All lanes: MKL2 antibody at 4.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 119, 43, 52, 115, 120 kDa
Observed band size: 119 kDa
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IHC image of CSB-PA891962LA01HU diluted at 1:500 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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