| Объем | 100 мкг |
| Синонимы | Annexin A5 (Anchorin CII) (Annexin V) (Annexin-5) (Calphobindin I) (CBP-I) (Endonexin II) (Lipocortin V) (Placental anticoagulant protein 4) (PP4) (Placental anticoagulant protein I) (PAP-I) (Thromboplastin inhibitor) (Vascular anticoagulant-alpha) (VAC-alpha), ANXA5, ANX5 ENX2 PP4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P08758 |
| Иммуноген | Recombinant Human Annexin A5 protein (2-320AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:1000 |
| Примечание | This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Cardiovascular, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Annexin A5 antibody at 2µg/ml
Lane 1: Hela whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA001846YA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate
All lanes: ANXA5 antibody at 1:3000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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IHC image of CSB-PA001846YA01HU diluted at 1:500 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA001846YA01HU diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: ANXA5 Antibody.