| Объем | 50 мкл |
| Синонимы | DNA replication licensing factor MCM3 (EC 3.6.4.12) (DNA polymerase alpha holoenzyme-associated protein P1) (P1-MCM3) (RLF subunit beta) (p102), MCM3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P25205 |
| Иммуноген | Recombinant Human DNA replication licensing factor MCM3 protein (1-295AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, ChIP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: MCM3 antibody at 4 µg/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: Jurkats whole cell lysate
Lane 6: U917 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 91, 96 kDa
Observed band size: 91 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA013591ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA013591ESR1HU at dilution of 1:100
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Chromatin Immunoprecipitation 293T (1.6*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-MCM3 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP013591HU) against the IRF-1 promoter.
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Наименование: MCM3 Antibody.