| Объем | 100 мкл |
| Синонимы | Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC, HIST1H2BE, HIST1H2BF, HIST1H2BG, HIST1H2BI, H2BFL, H2BFH, H2BFG, H2BFA, H2BFK |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P62807 |
| Иммуноген | Peptide sequence around site of Mono-methyl-Lys (12) derived from Human Histone H2B |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, ICC, ChIP, Recommended dilution: WB:1:100-1:1000, ICC:1:500-1:1000 |
| Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H2B type 1-C/E/F/G/I |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate
All lanes: HIST1H2BC antibody at 1:100
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
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Immunocytochemistry analysis of CSB-PA010403OA12me1HU diluted at 1:30 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA12me1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Наименование: Mono-methyl-HIST1H2BC (K12) Antibody.