| Объем | 100 мкл |
| Синонимы | Histone H1.0 (Histone H1') (Histone H1(0)) [Cleaved into: Histone H1.0, N-terminally processed], H1F0, H1FV |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P07305 |
| Иммуноген | Peptide sequence around site of Mono-methyl-Lys (101) derived from Human Histone H1.0. |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, ICC, IF, ChIP, Recommended dilution: WB:1:100-1:1000, ICC:1:1-1:10, IF:1:50-1:200 |
| Примечание | Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H1.0 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: H1F0 antibody at 1:50
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
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Immunofluorescence staining of HepG2 with CSB-PA010087OA101me1HU, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (CSB-PA0100870A101me1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Immunocytochemistry analysis of CSB-PA010087OA101me1HU diluted at 1:5 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: Mono-methyl-H1F0 (K101) Antibody.