MEF2A (Ab-312) AntibodyСпецификацияОбъем | 100 мкл | Синонимы | MEF2, Serum response factor-like protein 1, | Клональность | Polyclonal Antibody | Организм | Human | uniprot | Q02078 | Иммуноген | Peptide sequence around aa.310~314 (L-A-T-P-V) derived from Human MEF2A. | Источник | Rabbit | Видовая специфичность | Human, Mouse, Rat | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | Примечание | The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain<br/><br/>Kato Y, et al. (2000) J Biol Chem. 275(24): 18534-18540.<br/>Zhao M, et al. (1999) Mol Cell Biol. 19(1): 21-30.<br/><br/> | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | Форма | liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cardiovascular | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 3T3 cells using MEF2A(Ab-312) Antibody and the same antibody preincubated with blocking peptide.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEF2A(Ab-312) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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