| Объем | 100 мкг |
| Синонимы | DNA mismatch repair protein Mlh1 (MutL protein homolog 1), MLH1, COCA2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P40692 |
| Иммуноген | Recombinant Human DNA mismatch repair protein Mlh1 protein (350-519AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | DNA mismatch repair protein Mlh1 |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate
All lanes: MLH1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85, 59, 74 kDa
Observed band size: 85 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA17009A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA17009A0Rb at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA17009A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: MLH1 Antibody.