PECAM1 (Ab-713) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | CD31, CD31 antigen, EndoCAM, PEC1, PECA1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P16284 | | Иммуноген | Synthesized non-phosphopeptide derived from human PECAM-1 around the phosphorylation site of tyrosine 713 (T-V-Y(p)-S-E). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Induces susceptibility to atherosclerosis By similarity. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).<br/><br/>Tieming Zhao, J. Cell Biol., Jan 2001, 152: 65. <br/>SM Watt, Blood, Nov 1993, 82: 2649 - 2663. <br/>NETA ILAN, FASEB J, Feb 2001, 15: 362. <br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Cancer,Cardiovascular,Immunology,Signal transduction,Stem cells | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemistry analysis of paraffin-embedded human brain tissue using PECAM-1 (Ab-713) antibody.
 | Immunofluorescence analysis of HeLa cells, using PECAM-1 (Ab-713) antibody.
 | Western blot analysis of extracts from Jurkat cells, using PECAM-1 (Ab-713) antibody.
 | Western blot analysis of extracts from COS7 cells (Lane 2) and JK cells (Lane 3), using PECAM-1 (Ab-713) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: PECAM1 (Ab-713) Antibody.