| Объем | 100 мкл |
| Синонимы | N-alpha-acetyltransferase 20 (EC 2.3.1.254) (Methionine N-acetyltransferase) (N-acetyltransferase 5) (N-terminal acetyltransferase B complex catalytic subunit NAA20) (N-terminal acetyltransferase B complex catalytic subunit NAT5) (NatB complex subunit NAT5) (NatB catalytic subunit), NAA20, NAT5 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P61599 |
| Иммуноген | Recombinant Human N-alpha-acetyltransferase 20 protein (49-178AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IP, Recommended dilution: WB:1:1000-1:5000, IP:1:200-1:2000 |
| Примечание | Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: NAA20 antibody at 10µg/ml
Lane 1: Hela whole cell lysate
Lane 2: Rat brain tissue
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 21, 13 kDa
Observed band size: 21 kDa
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Immunoprecipitating NAA20 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA015472ESR2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA015472ESR2HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: NAA20 Antibody.