Объем | 50 мкл |
Синонимы | Cytoplasmic protein NCK1 (NCK adaptor protein 1) (Nck-1) (SH2/SH3 adaptor protein NCK-alpha), NCK1, NCK |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P16333 |
Иммуноген | Recombinant Human Cytoplasmic protein NCK1 protein (58-377AA) |
Источник | Rabbit |
Видовая специфичность | Human, Rat |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
Примечание | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity purified |
Области исследований | Epigenetics and Nuclear Signaling, Cancer, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Cytoplasmic protein NCK1 antibody at 4µg/ml + Rat liver tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43, 36 kDa
Observed band size: 43 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA015530DSR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA015530DSR2HU at dilution of 1:100
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