Объем | 50 мкг |
Синонимы | D-3-phosphoglycerate dehydrogenase (3-PGDH) (EC 1.1.1.95) (2-oxoglutarate reductase) (EC 1.1.1.399) (Malate dehydrogenase) (EC 1.1.1.37), PHGDH, PGDH3 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | O43175 |
Иммуноген | Recombinant Human D-3-phosphoglycerate dehydrogenase protein (2-251AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | PHGDH or 3-phosphoglycerate dehydrogenase is an enzyme belonging to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalysis the reaction 3-phosphoglycerate + NAD+ = 3-phosphonooxypyruvate + NADH during L-serine biosynthesis. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation and seizures. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | D-3-phosphoglycerate dehydrogenase protein |
Области исследований | Neuroscience, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: D-3-phosphoglycerate dehydrogenase antibody at 2µg/ml
Lane 1: EC109whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA03255A0Rb at dilution of 1:100
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Immunofluorescent analysis of MCF-7 cells using CSB-PA03255A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunofluorescent analysis of PC-3 cells using CSB-PA03255A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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