| Объем | 100 мкл |
| Синонимы | NEDD8-activating enzyme E1 regulatory subunit (Amyloid beta precursor protein-binding protein 1, 59 kDa) (APP-BP1) (Amyloid protein-binding protein 1) (Proto-oncogene protein 1), NAE1, APPBP1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13564 |
| Иммуноген | Recombinant Human NEDD8-activating enzyme E1 regulatory subunit protein (1-220AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | NEDD8-activating enzyme E1 regulatory subunit |
| Области исследований | Epigenetics and Nuclear Signaling, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: NAE1 antibody at 1.85µg/ml
Lane 1: Mouse liver tissue
Lane 2: Mouse kidney tissue
Lane 3: Mouse skeletal muscle tissue
Lane 4: 293T whole cell lysate
Lane 5: A431 whole cell lysate
Lane 6: K562 whole cell lysate
Lane 7: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 61, 60, 51 kDa
Observed band size: 61 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA613411ESR1HU at dilution of 1:100
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Наименование: NAE1 Antibody.