| Объем | 50 мкг |
| Синонимы | Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta), NCK2, GRB4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O43639 |
| Иммуноген | Recombinant Human Cytoplasmic protein NCK2 protein (1-380AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Tags & Cell Markers, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Cytoplasmic protein NCK2 antibody at 8µg/ml
Lane 1: Mouse brain tissue
Lane 2: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA015531HA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA015531HA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA015531HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: NCK2 Antibody.