Объем | 100 мкл |
Синонимы | Nucleolin (Protein C23), NCL |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P19338 |
Иммуноген | Recombinant Human Nucleolin protein (411-710AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200 |
Примечание | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Области исследований | Epigenetics and Nuclear Signaling, Tags & Cell Markers |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: NCL antibody at 8µg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa
![](http://www.cusabio.cn/images/antibody/6/CSB-PA015535DSR2HU-WB.jpg) |
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA015535DSR2HU at dilution of 1:100
![](http://www.cusabio.cn/images/antibody/2/CSB-PA015535DSR2HU-IHC-1.jpg) |
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