| Объем | 100 мкл |
| Синонимы | RNF53 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P38398 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 1524 (Y-P-S(p)-Q-E) derived from Human BRCA1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC,IHC:1:50-1:100 |
| Примечание | The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation<br/><br/>Lin SY, et al. (2004) Proc Natl Acad Sci U S A, 101(17): 6484-6489<br/>Zhang J, et al. (2004) Mol Cell Biol, 24(2): 708-718<br/>Beger C, et al. (2001) Proc Natl Acad Sci U S A, 98(1): 130-135<br/>Gardner K, et al. (2001) Breast Cancer Res, 3(1): 11-13<br/>Zheng L, et al. (2001) Proc Natl Acad Sci U S A, 98(17): 9587-9592<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser1524 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1(Phospho-Ser1524) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-BRCA1 (Ser1524) Antibody.