| Объем | 100 мкл |
| Синонимы | APAF-3, MCH6, RNCASP9, CASP-9, ICE-LAP6 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P55211 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.<br/><br/>Duan H., J. Biol. Chem. 271:16720-16724(1996).<br />Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).<br />Hadano S., Mamm. Genome 10:757-760(1999)<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr125 |
| Области исследований | Cancer,Cell biology,Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Наименование: Phospho-CASP9 (Thr125) Antibody.