| Объем | 100 мкл |
| Синонимы | CDC28, CDC2A, CDK1, Cyclin-dependent kinase 1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P06493 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine161 (T-Y-T(p)-H-E) derived from Human CDC2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.<br/><br/>Ukomadu C, et al.(2003) J Biol Chem, 278(7): 4840-6.<br/>Morris MC, et al.(2002)J Biol Chem, 277(26): 23847-53.<br/>Brown NR, et al.(1999)J Biol Chem, 274(13): 8746-56.<br/>Liu Y, et al.(2004)J Biol Chem, 279(6): 4507-14.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr161 |
| Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cell biology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Thr161) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2(Phospho-Thr161) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-CDK1 (Thr161) Antibody.