| Объем | 100 мкл |
| Синонимы | NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8) (Ubiquitin-like protein Nedd8), NEDD8 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q15843 |
| Иммуноген | Recombinant Human NEDD8 protein (1-81AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: NEDD8 antibody at 16µg/ml
Lane 1: Mouse heart tissue
Lane 2: Mouse spleen tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 9 kDa
Observed band size: 9 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA618018ESR1HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA618018ESR1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: NEDD8 Antibody.