Объем | 100 мкл |
Синонимы | GAT1, GATA1, GF-1, NF-E1, |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P15976 |
Иммуноген | Peptide sequence around phosphorylation site of serine 142 (R-L-S(p)-P-D) derived from Human GATA1. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
Примечание | <p>GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. <br /><br />Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672. <br />Trainor C D, et al. (1990) Nature. 343: 92-96. <br />Nichols K E, et al. (2000) Nat Genet. 24: 266-270.</p> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Ser142 |
Области исследований | Epigenetics and Nuclear Signaling,Developmental biology,Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HL60 cells untreated or treated with PMA using GATA1(Phospho-Ser142) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1(Phospho-Ser142) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using GATA1(Phospho-Ser142) Antibody.
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