| Объем | 50 мкг |
| Синонимы | Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9H9S0 |
| Иммуноген | Recombinant Human Homeobox protein NANOG protein (1-305AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200 |
| Примечание | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Developmental biology, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Ntera-2 whole cell lysate
All lanes: NANOG antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35, 33 kDa
Observed band size: 35 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA888008LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA888008LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA888008LA01HU at dilution of 1:100
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Наименование: NANOG Antibody.