| Объем | 100 мкг |
| Синонимы | NPC intracellular cholesterol transporter 1 (Niemann-Pick C1 protein), NPC1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O15118 |
| Иммуноген | Recombinant Human NPC intracellular cholesterol transporter 1 protein (23-269AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
| Примечание | Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | NPC intracellular cholesterol transporter 1 |
| Области исследований | Neuroscience, Cardiovascular, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: NPC1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 143, 108 kDa
Observed band size: 108 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA890689LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA890689LA01HU at dilution of 1:100
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Наименование: NPC1 Antibody.