| Объем | 50 мкг |
| Синонимы | Nephrin (Renal glomerulus-specific cell adhesion receptor), NPHS1, NPHN |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O60500 |
| Иммуноген | Recombinant Human Nephrin protein (23-257AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015988LA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: NPHS1 antibody at 5.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 135, 131 kDa
Observed band size: 135 kDa
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Immunofluorescence staining of HepG2 cells with CSB-PA015988LA01HU at 1:125, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: NPHS1 Antibody.