| Объем | 100 мкг |
| Синонимы | Ribosyldihydronicotinamide dehydrogenase [quinone] (EC 1.10.5.1) (NRH dehydrogenase [quinone] 2) (NRH:quinone oxidoreductase 2) (Quinone reductase 2) (QR2), NQO2, NMOR2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P16083 |
| Иммуноген | Recombinant Human Ribosyldihydronicotinamide dehydrogenase [quinone] protein (1-231AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Ribosyldihydronicotinamide dehydrogenase [quinone] |
| Области исследований | Cancer, Cell biology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: K562 whole cell lysate, Mouse liver tissue
All lanes: NQO2 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA016040LA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA016040LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating NQO2 in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA016040LA01HU in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA016040LA01HU (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
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Наименование: NQO2 Antibody.