| Объем | 100 мкл |
| Синонимы | MACS, 80K-L, PKCSL, PRKCSL |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P29966 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 170 (G-F-S(p)-F-K) derived from Human MARCKS. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. <br /><br />Ramsden, J.J. (2000) Int. J. Biochem. Cell Biol. 32, 475-479. <br />Graff, J. M. et al. (1989) J. Biol. Chem. 264, 21818-21823. <br />Hartwig, J. H. et al. (1992) Nature 356, 618-622. <br />Thelen, M. et al. (1991) Nature 351, 320-322.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser170 |
| Области исследований | Cardiovascular,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MCF cells untreated or treated with EGF using MARCKS(phospho-Ser170) Antibody.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS(Phospho-Ser170) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Western blot analysis of extracts from 3T3 cells, treated with serum or calf intestinal phosphatase (CIP), using MARCKS (phospho-Ser170) Antibody.
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Наименование: Phospho-MARCKS (Ser170) Antibody.