| Объем | 100 мкл |
| Синонимы | NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | P25963 |
| Иммуноген | A synthesized peptide derived from human Phospho-NFKBIA (S32) |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Signal Transduction |
| Аббревиатура | NF-kappa-B inhibitor alpha |
| Примечание | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in:A549 whole cell lysate(treated with Calyculin A or not)
All lanes:Phospho-NFKBIA antibody at 1.07µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 KDa
Observed band size: 39 KDa
 |
Immunofluorescence staining of A549 cells with CSB-RA015761A32phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
 |
| | |
Наименование: Phospho-NFKBIA (S32) Antibody.