| Объем | 100 мкл |
| Синонимы | p21-activated kinase 1, PAK-1, p65-PAK, Alpha-PAK, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13153/Q13177/O75914 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 423 /402/421 (R-S-T(p)-M-V) derived from Human PAK1/PAK2/PAK3. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, IHC, IF,IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. <br /><br />Parsons M, et al. (2005) Mol Cell Biol , 25:1680-1695. <br />Jung JH, et al. (2005) J Biol Chem, 280:40025-40031. <br />Lampugnani MG, et al. (2002) Mol Biol Cell , 13: 1175-1189 <br />Wang RA, et al. (2002) EMBO J, 21: 5437 - 5447.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr423/Thr402/Thr421 |
| Области исследований | Cancer,Cell biology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody.
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Наименование: Phospho-PAK1/PAK2/PAK3 (Thr423/Thr402/Thr421) Antibody.