| Объем | 100 мкл |
| Синонимы | KPCT, PKC-theta, PKCQ, PRKCQ, PRKCT |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q04759 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 676 (R-L-S(p)-F-A) derived from Human PKCth. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. <br /><br />Kristof Van Kolen, et al. (2006) FEBS J , 273: 1843 - 1854. <br />Martin Villalba, et al. (2002) J. Cell Biol , 157: 253. <br />Jie Zhang, et al. (2004) J. Biol. Chem , 279: 22118 - 22123. <br />Castro AF, et al. (1998) Am J Physiol Cell Physiol, 275: C113 - C119. </p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser676 |
| Области исследований | Cancer,Metabolism,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCth(Phospho-Ser676) Antibody.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser676) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using PKCth(Phospho-Ser676) Antibody.
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Western blot analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), using PKC? (Phospho-Ser676) Antibody.
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Наименование: Phospho-PRKCQ (Ser676) Antibody.