| Объем | 50 мкл |
| Синонимы | Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3, PTPN11, PTP2C, SHPTP2 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | Q06124 |
| Иммуноген | A synthesized peptide derived from human Phospho-PTPN11 (Y542) |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IP, Recommended dilution: WB:1:500-1:5000, IP:1:200-1:1000 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Signal Transduction |
| Аббревиатура | Tyrosine-protein phosphatase non-receptor type 11 |
| Примечание | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426). |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in:Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not)
All lanes:Phospho-PTPN11 antibody at 0.65µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 KDa
Observed band size: 68 KDa
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Immunoprecipitating Phospho-PTPN11 in Hela whole cell lysate treated with Pervanadate
Lane 1: Rabbit control IgG(1µg)instead of CSB-RA019025A542phHU in Hela whole cell lysate treated with Pervanadate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA019025A542phHU(3µg)+ Hela whole cell lysate treated with Pervanadate(1mg)
Lane 3: Hela whole cell lysate treated with Pervanadate(20µg)
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Наименование: Phospho-PTPN11 (Y542) Antibody.