Phospho-RAF1 (S259) Antibody

Спецификация

Объем	100 мкл
Синонимы	RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF
Тип антител	Recombinant Antibody
Species	Human
UniProt ID	P04049
Иммуноген	A synthesized peptide derived from human Phospho-RAF1 (S259)
Видовая специфичность	Human
Применение	ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Клональность	Monoclonal
Изотип	Rabbit IgG
Коньюгат	Non-conjugated
Буффер	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Форма	Liquid
Хранение	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки	Affinity-chromatography
Области исследований	Signal Transduction
Аббревиатура	RAF proto-oncogene serine/threonine-protein kinase
Примечание	Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
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Western Blot Positive WB detected in:Hela whole cell lysate(treated with Calyculin A or EGF) All lanes:Phospho-RAF1 antibody at 1.18µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 KDa Observed band size: 73 KDa
IHC image of CSB-RA019284A259phHU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-RA019284A259phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Информация для заказа

Область использования: Рекомбинантные антитела Cusabio	Производство:	Cusabio
	Метод:	Антитела
	Объем:	100 мкл
	Кат. номер:	CSB-RA019284A259phHU
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Phospho-RAF1 (S259) Antibody. Примечание: дополнительная информация (на английском языке).