Объем | 50 мкл |
Синонимы | Protein NEDD1 (Neural precursor cell expressed developmentally down-regulated protein 1) (NEDD-1), NEDD1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q8NHV4 |
Иммуноген | Recombinant Human Protein NEDD1 protein (411-660AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Protein NEDD1 |
Области исследований | Epigenetics and Nuclear Signaling, Cell biology, Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: NEDD1 antibody at 2.66µg/ml
Lane 1: Mouse brain tissue
Lane 2: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 72, 63, 73 kDa
Observed band size: 72 kDa
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Immunohistochemistry of paraffin-embedded human glioma using CSB-PA822820DSR2HU at dilution of 1:100
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Immunoprecipitating NEDD1 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA822820DSR2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA822820DSR2HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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