| Объем | 50 мкг |
| Синонимы | Nuclear pore complex protein Nup85 (85 kDa nucleoporin) (FROUNT) (Nucleoporin Nup75) (Nucleoporin Nup85) (Pericentrin-1), NUP85, NUP75 PCNT1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9BW27 |
| Иммуноген | Recombinant Human Nuclear pore complex protein Nup85 protein (201-500AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Tags & Cell Markers |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Mouse lung tissue
All lanes: NUP85 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 53, 70 kDa
Observed band size: 76, 70 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA874821LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA874821LA01HU at dilution of 1:100
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Immunofluorescent analysis of MCF-7 cells using CSB-PA874821LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: NUP85 Antibody.