Phospho-STAT1 (Tyr701) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | CANDF7, ISGF-3, STAT91 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P42224 | | Иммуноген | Peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y(p)-I-K) derived from Human STAT1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 | | Примечание | <p>Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. <br /><br />Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. <br />Durbin J E, et al. (1996) Cell. 84: 443-450. <br />Meraz M A, et al. (1996) Cell. 84: 431-442. <br />Wakao H, et al. (1994) EMBO J. 13: 2182-2191. <br />Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258 <br />Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Tyr701 | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from MEF cells untreated or treated with interferon-96
 | Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using STAT1 (Phospho-Tyr701) Antibody.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA926759 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-STAT1 (Tyr701) Antibody.
Примечание: дополнительная информация (на английском языке). |
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