| Объем | 100 мкл |
| Синонимы | Spleen tyrosine kinase |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P43405 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y(p)-E- P) derived from Human syk. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion <br/><br/>Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.<br/>Turner, M. et al. (2000) Immunol. Today 21, 148-154.<br/>Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.<br/>Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr323 |
| Области исследований | Cardiovascular,Immunology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from JK cells untreated or treated with H2O2 for the indicated times, using syk(phospho-Tyr323) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk(Phospho-Tyr323) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using syk(phospho-Tyr323) Antibody.
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Наименование: Phospho-SYK (Tyr323) Antibody.