Объем | 100 мкг |
Синонимы | Protein naked cuticle homolog 1 (Naked-1) (hNkd) (hNkd1), NKD1, NKD |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q969G9 |
Иммуноген | Recombinant Human Protein naked cuticle homolog 1 protein (269-384AA) |
Источник | Rabbit |
Видовая специфичность | Human, Rat |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
Примечание | Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Protein naked cuticle homolog 1 |
Области исследований | Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Rat heart tissue
All lanes: NKD1 antibody at 1.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
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Immunofluorescence staining of HepG2 cells with CSB-PA853369LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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