Объем | 50 мкл |
Синонимы | Glycylpeptide N-tetradecanoyltransferase 2 (EC 2.3.1.97) (Myristoyl-CoA:protein N-myristoyltransferase 2) (NMT 2) (Peptide N-myristoyltransferase 2) (Type II N-myristoyltransferase), NMT2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | O60551 |
Иммуноген | Recombinant Human Glycylpeptide N-tetradecanoyltransferase 2 protein (1-120AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Glycylpeptide N-tetradecanoyltransferase 2 |
Области исследований | Cancer, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: NMT2 antibody at 4.99µg/ml
Lane 1: Human placenta tissue
Lane 2: K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA015901DSR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA015901DSR2HU at dilution of 1:100
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Immunoprecipitating NMT2 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA015901DSR2HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA015901DSR2HU (4µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)
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