PIP5K1A AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha (PIP5K1-alpha) (PtdIns(4)P-5-kinase 1 alpha) (EC 2.7.1.68) (68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha) (Phosphatidylinositol 4-phosphate 5-kinase type I alpha) (PIP5KIalpha), PIP5K1A | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q99755 | | Иммуноген | Recombinant Human Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha protein (293-562AA) | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 | | Примечание | Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen Affinity Purified | | Абревеатура | Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha | | Области исследований | Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot
All lanes: PIP5K1A antibody at 4.12µg/ml
Lane 1: Hela whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: Mouse heart tissue
Lane 4: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 63, 57, 62, 59 kDa
Observed band size: 63 kDa
 | Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA859110ESR1HU at dilution of 1:100
 | Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA859110ESR1HU at dilution of 1:100
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Наименование: PIP5K1A Antibody.