| Объем | 100 мкг |
| Синонимы | Rho guanine nucleotide exchange factor 7 (Beta-Pix) (COOL-1) (PAK-interacting exchange factor beta) (p85), ARHGEF7, COOL1 KIAA0142 P85SPR PAK3BP PIXB |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14155 |
| Иммуноген | Recombinant Human Rho guanine nucleotide exchange factor 7 protein (1-250AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IF, IP, Recommended dilution: IF:1:200-1:500, IP:1:200-1:2000 |
| Примечание | Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions By similarity. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescence staining of Hela cells with CSB-PA01445A0Rb at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating ARHGEF7 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA01445A0Rb in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA01445A0Rb (6µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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Наименование: ARHGEF7 Antibody.