| Объем | 100 мкл |
| Синонимы | Programmed cell death protein 4 (Neoplastic transformation inhibitor protein) (Nuclear antigen H731-like) (Protein 197/15a), PDCD4, H731 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q53EL6 |
| Иммуноген | Recombinant Human Programmed cell death protein 4 protein (1-260AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IP, Recommended dilution: WB:1:1000-1:5000, IP:1:200-1:2000 |
| Примечание | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA . |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Programmed cell death protein 4 antibody at 5µg/ml
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 52, 51 kDa
Observed band size: 52 kDa
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Immunoprecipitating PDCD4 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA703610DSR1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA703610DSR1HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: PDCD4 Antibody.