Объем | 50 мкл |
Code NO# | 14D8C2 |
Синонимы | Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274), CD274, B7H1 PDCD1L1 PDCD1LG1 PDL1 |
Тип антител | Monoclonal Antibody |
Видовая специфичность | Human |
Uniport ID | Q9NZQ7 |
Иммуноген | Recombinant Human Programmed cell death 1 ligand 1 protein (19-238AA) |
Источник | Mouse |
Видовая специфичность 1 | Human |
Применение | ELISA, WB, IHC, IF, FC, Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200 |
Примечание | Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077) |
Изотип | IgG2b |
Метод очистки | >95%, Protein G purified |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Области исследований | Immunology, Cancer |
Станица на сайте производителя | ссылка |
Western Blot
Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate
All lanes: PD-L1 antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 34, 21 kDa
Observed band size: 55, 70 kDa
![](http://www.cusabio.cn/images/antibody/Monoclonal/CSB-MA878942A1m-WB-1.jpg) |
IHC image of CSB-MA878942A1m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
![](http://www.cusabio.cn/images/antibody/Monoclonal/CSB-MA878942A1m-IHC-1.jpg) |
IHC image of CSB-MA878942A1m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
![](http://www.cusabio.cn/images/antibody/Monoclonal/CSB-MA878942A1m-IHC-2.jpg) |
Immunofluorescence staining of 293 cells with CSB-MA878942A1m at 1:150, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
![](http://www.cusabio.cn/images/antibody/Monoclonal/CSB-MA878942A1m-IF-1.jpg) |
Immunofluorescence staining of A549 cells with CSB-MA878942A1m at 1:150, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
![](http://www.cusabio.cn/images/antibody/Monoclonal/CSB-MA878942A1m-IF-2.jpg) |
Immunofluorescence staining of Hela cells with CSB-MA878942A1m at 1:150, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
![](http://www.cusabio.cn/images/antibody/Monoclonal/CSB-MA878942A1m-IF-3.jpg) |
Overlay histogram showing 293 cells stained with CSB-MA878942A1m (red line) at 1:300. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
![](http://www.cusabio.cn/images/antibody/Monoclonal/CSB-MA878942A1m-FC-1.jpg) |
Overlay histogram showing A549 cells stained with CSB-MA878942A1m (red line) at 1:300. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
![](http://www.cusabio.cn/images/antibody/Monoclonal/CSB-MA878942A1m-FC-2.jpg) |
Overlay histogram showing Hela cells stained with CSB-MA878942A1m (red line) at 1:300. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
![](http://www.cusabio.cn/images/antibody/Monoclonal/CSB-MA878942A1m-FC-3.jpg) |
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