| Объем | 100 мкл |
| Синонимы | ADDA, Erythrocyte adducin alpha subunit, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P35611 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described. <br/><br/>Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30, 102(35): 12407-12<br/>Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01, 332(2): 347-51<br/>Barkalow KL, et al. J Cell Biol 2003 May 12, 161(3): 557-70<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser726 |
| Области исследований | Tags & Cell Markers,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells using ADD1(Phospho-Ser726) Antibody and the same antibody preincubated with blocking peptide.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1(Phospho-Ser726) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using ADD1(Phospho-Ser726) Antibody.
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Наименование: Phospho-ADD1 (Ser726) Antibody.