Phospho-AKT1 (Ser473) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | C-AKT, PKB, PKB-alpha, RAC, RAC-PK-alpha,akt s473,akt 473,akt ser473 antibody | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P31749 | | Иммуноген | Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from Human Akt. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 | | Примечание | <p>General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. <br /><br />Baudhuin LM, et al. (2004) FASEB J Feb, 18(2): 341-3. <br />Min YH, et al. (2004) Cancer Res, 64(15): 5225-31. <br />Feng J, et al. (2004) J Biol Chem, 279(34): 35510-7.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Ser473 | | Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Phospho-Ser473) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Western blot analysis of extracts from 293 cells, treatedwith EGF or calf intestinal phosphatase (CIP), using Akt(Phospho-Ser473) Antibody.
 | Western blot analysis of extracts from SK-BR-3 cells,treated with insulin and EGF, and pretreated with U0126and LY294002 cells using Akt (Phospho-Ser473) Antibody.
 | Immunohistochemical analysis of paraffinembeddedhuman Lung carcinoma tissue usingAkt (Phospho-Ser473) Antibody.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA573922 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-AKT1 (Ser473) Antibody.
Примечание: дополнительная информация (на английском языке). |
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